After I found a transparent spot following washing the penis with a wet tissue, changing clothes, and performing ablution for Fajr prayer, is my prayer not accepted, knowing that I suffer greatly from this, and it causes me obsessive thoughts and distress?

If a transparent drop appears after the prayer, your prayer is valid; because the impurity is attributed to the nearest possible time. This drop is considered to have exited after the prayer, and it may be pure water from ablution unless you are certain of its impurity.