Is it obligatory to make up prayers, fasting, and acts of worship missed due to ignorance of the difference between madhy (pre-seminal fluid) and maniy (seminal fluid) and other discharges and their rulings?

There is no obligation to make up acts of worship unless one is certain that a condition for their validity has been violated; doubt does not affect them. If a female worshipper is certain that she prayed without purification, there is a difference of opinion regarding the obligation to make up the prayer, but the more cautious approach is to make it up. As for fasting, it is valid in all cases, and there is no obligation to make up any part of it.