Is it necessary to separate from one's wife given her family's attempts to cause discord between the spouses, and what is the religious ruling on the wife's request for divorce in this situation?

There should be no haste in divorce as long as the problem can be solved through legitimate means. Therefore, we advise you to communicate with your wife's family to understand the reasons for their attempt to separate you, and try to resolve them through dialogue. If they do not respond, they are sinful for their endeavor to corrupt the relationship between you, which is one of the greatest prohibitions. You may prevent your wife from going to them if this is their situation. Her duty is to obey you, as your obedience takes precedence over the obedience of her parents when there is a conflict. If she insists on pursuing what her family demands, she is defiant (nashiz).