Is it permissible to use glasses found two and a half years ago in public transportation, after donating their value based on a previous fatwa, or must they be advertised despite the passage of this period?

It was obligatory for you to publicize the found item for a full year. Since you neglected this due to an excuse, we hope there is no sin upon you. You can publicize it now, and if you do not find its owner, there is no harm for you in benefiting from it. However, if you strongly believe that publicizing it now will be fruitless, then give the remaining value in charity and benefit from it. If you find the owner of the glasses and return them to him, the reward of charity will be yours, unless the owner chooses the reward of charity for himself.