Is the broker entitled to claim the brokerage fee mentioned in the check, especially since he requested the amount be transferred to his wife, did not mention the company he claims is demanding it from him, and alleged not receiving the brokerage fee from the buyer, who confirmed paying it to other individuals?

The reference for determining the broker's fee is the agreement between the broker and the contracting parties. If there is no agreement, then the reference is the prevailing custom in real estate brokerage, and the brokerage fee will be according to the customary amount, and on whom it is binding, and who receives it. If an agreement is reached contrary to custom, there is no harm, otherwise, it is not permissible to take money without willing consent.